Assessment of Regenerative Response of Early Sugarcane Varieties under In vitro Condition

With the aim to assess the regenerative response of six mid late sugarcane clones under in vitro conditions with four different types of growth media and the research was conducted in Sugarcane Hi-tech Laboratory, SRI, DRPCAU, Pusa, Bihar. Shoot apex explant culture of these cultivars on media M1 (MS basal) and M2 (ms basal + 0.5 mgl1 IAA + 0.5 mgl1 + 0.5mgl1 KIN) suggested no definite role of medium on establishment of cultures. Shoot apex culture of the six selected clones on media M3 (MS basal +0.1 mgl1 IAA + 2.0 mgl1 BAP + 1.0 mgl1 KIN) and M4 (MS basal +0.1 mgl1 IAA + 2.0 mgl1 BAP + 1.0 mgl1 KIN) resulted in shoot proliferation and elongation. A genotypic difference for all tissue culture responses were found in the six selected cultivars. CoP16437 has the best response among these six cultivars, while CoP9301 has the lowest response.